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anti rabbit secondary antibodies  (Proteintech)


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    Structured Review

    Proteintech anti rabbit secondary antibodies
    Anti Rabbit Secondary Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 9023 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hrp+conjugated+goat+anti+rabbit+secondary+antibody/Goat+anti-rabbit+IgG+(H%2BL)%2C+HRP+conjugate/pmc13651863-280-20-27
    Average 98 stars, based on 9023 article reviews
    anti rabbit secondary antibodies - by Bioz Stars, 2026-10
    98/100 stars

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    other:

    Article Title: Functionalized Magnetic Hydrogel Encapsulation of Human Dental Follicle Stem Cells under a Static Magnetic Field Enhances Multi-site Bone Regeneration
    Article Snippet: After 14 days of osteogenic induction, proteins were extracted using ice-cold Page 13 of 59 https://mc.manuscriptcentral.com/rb Manuscripts submitted to Regenerative Biomaterials 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 on 15 M arch 2026 RIPA lysis buffer (Solarbio) and quantified via the BCA assay

    Incubation:

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation
    Article Snippet: Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 °C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China). .. Signal intensity was quantified using ImageJ software (version 1.53).

    Article Title: All-trans retinoic acid modulates proliferation and apoptosis of secondary hair follicle–dermal papilla cells in cashmere goats via the TGF- β 2/Smad2/3 pathway
    Article Snippet: The following antibodies were used: rabbit monoclonal anti-Bcl-2 (1:1,000; #3498; Cell Signaling Technology, Danvers, MA, United States), rabbit polyclonal anti-Bax (1:5,000; 50,599-2-Ig; Proteintech, Rosemont, IL, United States), rabbit polyclonal anti-TGF- β 2 (1:1,000; 19,999-1-AP; Proteintech), rabbit monoclonal anti-phospho-Smad2 (1:1,000; #18338; Cell Signaling Technology), rabbit polyclonal anti-Smad2 (1:2,000; 12,570-1-AP; Proteintech), rabbit polyclonal anti-Smad3 (1:1,000; 30,130-1-AP; Proteintech), rabbit polyclonal anti-phospho-Smad3 (1:1,000; YP0363; Immunoway), and rabbit polyclonal anti-GAPDH (1:5,000; 10,494-1-AP; Proteintech). .. After washing with TBST, membranes were incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:1,000; SA00001-2; Proteintech) for 1 h at room temperature. .. Protein bands were visualized using enhanced chemiluminescence reagent (Abbkine) and a Tanon 5,200 imaging system (Tanon, Shanghai, China).

    Article Title: Ginsenoside Rh4 Triggers Ferroptosis in Lung Cancer: Targeting KEAP1 / NRF2 / HO-1 and Remodeling Gut Microbiota for Butyrate-Mediated ATF3 Activation.
    Article Snippet: Primary antibodies against FTH1 (Sangon Biotech, Shanghai, China), GPX4 (Sangon Biotech, Shanghai, China), HO-1 (Proteintech, Wuhan, China), KEAP1 (Proteintech, Wuhan, China), NRF2 (Proteintech, Wuhan, China), SLC40A1 (Proteintech, Wuhan, China), SLC7A11 (Sangon Biotech, Shanghai, China), TFRC (Proteintech, Wuhan, China), and β-actin (Proteintech, Wuhan, China) were incubated with the membrane at 4 ◦C overnight. .. Following an hour of room temperature incubation with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech, Wuhan, China), protein bands were identified using a ChemiScope 5300 (Clinx Science Instruments Co., Ltd, Shanghai, China). .. Signal intensity was quantified using ImageJ software (version 1.53).

    Article Title: Glucose-driven TRIB3 enhances the tumorigenic potential of colon cancer via the PI3K/AKT pathway
    Article Snippet: The sections were then treated with 3% hydrogen peroxide for 15 min at room temperature to block endogenous peroxidase activity, followed by blocking with 5% goat serum (cat. no. 16210064; Gibco; Thermo Fisher Scientific, Inc.) for 30 min at room temperature. .. Subsequently, the sections were incubated overnight at 4°C with TRIB3 antibody (1:400; cat. no. ab137526; Abcam), followed by incubation with HRP-conjugated goat anti-rabbit secondary antibody (1:500; cat. no. SA00001-2; Proteintech Group, Inc.) for 1 h at room temperature. .. Immunolabeling was performed using a DAB detection kit (cat. no. ZLI-9019; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.).

    Article Title: GBP2 promotes podocyte pyroptosis and contributes to the pathogenesis of pediatric lupus nephritis.
    Article Snippet: The membranes were blocked for 2 h at room temperature with 5% skimmed milk (Beyotime) in TBST, followed by an overnight incubation at 4 °C with primary antibody against GBP2 (Immunoway Biotechnology, Suzhou, China; YN1744; 1:1000), AIM2 (Immunoway Biotechnology;YM8846; 1:2000), Caspase-1 (Immunoway Biotechnology;YM9369, 1:4000), GSDMD (Nature Biosciences, Beijing, China; A22998; 1:2000), and GAPDH (Share-Bio, Shanghai, China; SB-AB2000; 1:10000) as loading control. .. Membranes were washed three times for 10 minutes each in TBST and incubated for 1 h at room temperature with an HRP-conjugated goat anti-rabbit secondary antibody (Proteintech Group, Rosemont, IL, USA; SA00001-2) diluted at 1:10,000. .. Protein bands were visualized using HRP chemiluminescent substrate (Biosharp Life Sciences, Hefei, China; BL520A) and quantified using ImageJ software (v1.54g).

    Article Title: rsRNA-28S-21 promotes endometrial cancer by regulating ferroptosis through the NSUN5-FTH1/FTL pathway.
    Article Snippet: rRNA-derived small RNAs (rsRNAs), processed from ribosomal RNA, represent a novel subclass of small noncoding RNAs.. Studies have demonstrated their involvement in cancer progression; however, their role in endometrial carcinoma remains unclear.. In this study, RIP-seq was performed to analyze NSUN5-bound rsRNAs in EC cells, and qRT-PCR was used to quantify rsRNA-28S-21 expression.



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